ABSTRACT
Objective To explore the potential mechanism of Epimedium and Morinda officinalis in the treatment of Corona Virus Disease 2019 (COVID-19) with deficiency of lung and kidney by network pharmacology. Methods The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform was used to search the compounds in Epimedium and Morinda, and the active components and targets were screened. Uniprot database was used for standardization, and the genes corresponding to the targets were searched. GeneCards database was used to obtain the targets of COVID-19 with deficiency of lung and kidney. The drug-compound-target network was constructed by the software of Cycloscape3. 7. 2, protein-protein interaction (PPI) network was constructed and analyzed by using Search Tool for the Retrieval of Interacting Genes (STRING) database. Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) path analysis were conducted by the software of Funrich 3. 1. 3. Results Epimedium and Morinda officinalis had 41 effective targets in the treatment of COVID-19. There were 2 831 targets associated with COVID-19 with deficiency of lung and kidney, and the key targets were IL6, VEGFA,CASP3, HIF1A, CCND1 9 EGFR etc. A total of 802 GO enrichment entries and 125 KEGG pathways were obtained, mainly involving PI3K-Akt signaling pathway, lipid and atherosclerosis, Kaposi sarcoma-associated herpesvirus infection, microRNAs in cancer, human cytomegalovirus infection, Epstein-Barr virus infection, etc. Conclusion The treatment of COVID-19 with deficiency of lung and kidney is the result of multi-target and multi-channel interaction, and it can be used as an adjuvant therapy for COVID-19 with deficiency of lung and kidney, which provides certain theoretical basis for expanding the clinical application of Chuankezhi injection. (English) [ABSTRACT FROM AUTHOR] 目的 运用网络药理学方法探讨淫羊藿-巴戟天治疗新型冠状病毒感染(新冠感染)肺肾两虚型 的潜在作用机制。方法 通过中药系统药理学数据库与分析平台检索淫羊藿、巴戟天含有的化合物,筛选其活 性成分及其作用靶点;运用 Uniprot数据库进行标准化处理,查询靶点对应的基因;通过 GeneCards数据库获 取新冠感染肺肾两虚型的相关靶标;采用 Cytoscape3.7.2软件构建药物-化合物-靶点网络,STRING 数据库进 行蛋白质相互作用(PPI)网络的构建与分析;通过 Funrich3.1.3软件进行基因本体(GO)富集分析和京都基因 与基因组百科全书(KEGG)通路分析。结果 获得淫羊藿-巴戟天治疗新冠感染的共41个有效靶点,新冠感染 肺肾两虚型相关靶点2831个,关键靶点有白细胞介素-6(IL-6)、血管内皮生长因子 A(VEGFA)、胱天蛋白酶3 (CASP3)、缺氧诱导因子1α(HIF1α)、细胞周期蛋白 D1(CCND1)、人类表皮生长因子受体(EGFR)等;获得802 个 GO 富集条目和125条 KEGG 通路,主要涉及磷酸肌醇-3激酶/蛋白激酶 B 信号通路、脂质核动脉粥样硬 化、卡波西氏肉瘤疱疹病毒感染、肿瘤相关 mRNA、人巨细胞病毒感染、艾巴氏病毒感染等。结论 淫羊藿-巴 戟天治疗新冠感染肺肾两虚型是多靶点、多途径相互作用的结果,可作为新冠感染肺肾两虚型的辅助治疗方 法,为扩展喘可治注射液的临床应用提供一定理论依据。 (Chinese) [ABSTRACT FROM AUTHOR] Copyright of Journal of Modern Medicine & Health is the property of Journal of Modern Medicine & Health and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)